Protein crystallization: virtual screening and optimization. DeLucas, L. J. et al. Progress in Biophysics and Molecular Biology 88 (2005) 285–309.
Relation between the Solubility of Proteins in Aqueous Solutions and the Second Virial Coefficient of the Solution. Haas, C. et al. J. Phys. Chem. B (1999) 103, 2808-2811.
Correlation between the osmotic second virial coefficient and solubility for equine serum albumin and ovalbumin. Demoruelle, K., et al. Acta Cryst. (2002) D58, 1544-1548.
High-Throughput Self-Interaction Chromatography: Applications in Protein Formulation Prediction. Johnson, D. H., et al. Pharmaceutical Research (2009) 26, 296-305.
Protein solubilization: A novel approach, Johnson, D. H., et al. Journal of Chromatography B (2014) 971, 99–106.
Applications of the second virial coefficient: protein crystallization and solubility. Wilson, W. W. and DeLucas, L. J. Acta Cryst. (2014) F70, 543–554.
Early Assessment of Pharmaceutical Formulations using OptiPharma Kit: A case study on Proprietary Pharmaceutical Protein
Keywords: Protein Solubilization, Pharmaceutical Formulation, Formulation Development, Protein Aggregation, Protein Physical Stability Our previous blog describes the use of OptiPharma kit on α-chymotrypsin, commercially available protein. Here we are presenting a...
Identifying Pharmaceutical Excipients and Solution Conditions for α-Chymotrypsin using OptiPharma Kit
Formulation development is one of the critical steps in developing a protein as a therapeutic product. Maintaining the integrity of purified protein during pharmaceutical processing, storage, handling, and delivery to patient is a major challenge. There are many...
A major hurdle for biologics development is the determination of a formulation that keeps a protein soluble. Soluble Biotech OptiSol Protein Solubilization Screening Kit (96-well format) contains a systematically varied array of buffers (from pH 3 to pH 10) and a...